Protein oxidation and carbonylation processes using proteomics and mass spectrometry techniques

The research topic is aimed at the identification and characterisation of new pharmacological targets and biomarkers of oxidative and carbonyl-based degenerative processes. Research in this area focused on the development and application of LC-MS/MS and immunohistochemical methodologies for the identification of post-translational modifications of peptides and proteins in different biological matrices as early biomarkers of oxidative/carbonyl damage.

Pubblicazioni

• Altomare A., Brioschi M., Eligini S., Bonomi A., Zoanni B., Iezzi A., Jemos C., Porro B., D’Alessandra Y., Guarino A., Omodeo Salè E., Aldini G., Agostoni P., Banfi C. N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin. Antioxidants 2022 Sep 6;11(9):1758. doi: 10.3390/antiox11091758.
• Altomare A., Baron G., Giannazza E., Banfi C., Carini M., Aldini G. Lipid peroxidation derived reactive carbonyl species in free and conjugated forms as an index of lipid peroxidation: limits and perspectives. Redox Biol. 2021 Jun:42:101899. doi: 10.1016/j.redox.2021.101899.
• Altomare A., Baron G., Balbinot M., Pedretti A., Zoanni B., Brioschi M., Agostoni P., Carini M., Banfi C., Aldini G. In-depth age and ale profiling of human albumin in heart failure: Ex vivo studies. Antioxidants 2021 Feb 27;10(3):358. doi: 10.3390/antiox10030358.
• Mol M., Degani G., Coppa C., Baron G., Popolo L., Carini M., Aldini G., Vistoli G., Altomare A. Advanced lipoxidation end products (ALEs) as RAGE binders: Mass spectrometric and computational studies to explain the reasons why. Redox Biol. 2019 May;23:101083. doi: 10.1016/j.redox.2018.101083.
• Aldini G,. Regazzoni L., Orioli M., Rimoldi I., Facino R.M., Carini M. A tandem MS precursor-ion scan approach to identify variable covalent modification of albumin Cys34: A new tool for studying vascular carbonylation. J Mass Spectrom. 2008 Nov;43(11):1470-81. doi: 10.1002/jms.1419.